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Image Search Results
Journal: PLoS ONE
Article Title: The Integrin-Linked Kinase-PINCH-Parvin Complex Supports Integrin αIIbβ3 Activation
doi: 10.1371/journal.pone.0085498
Figure Lengend Snippet: αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and β-actin are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.
Article Snippet: The membranes were incubated with either one of the following primary Abs: 0.125 μg/ml mouse mAb specific for ILK, 3/ILK (BD Biosciences), 0.25 μg/ml mouse mAb specific for PINCH, 49/PINCH (BD Biosciences), rabbit polyclonal Ab specific for α-parvin (IgG fraction; 1:3,000) (Sigma-Aldrich), 1 μg/ml mouse mAb specific for β-parvin, 11A5 (Millipore, Temecula, CA), mouse mAb specific for talin, 8D4 (ascites fluid; 1:2,000) (Sigma-Aldrich), rabbit polyclonal Ab specific for kindlin-2 (IgG fraction; 1:1,000) (ProteinTech Group, Chicago, IL), 0.5 μg/ml rabbit polyclonal Ab specific for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), FL-335 (Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated rabbit polyclonal Ab specific for
Techniques: Transfection, Negative Control, SDS Page, Activation Assay, Binding Assay, Standard Deviation