hrp conjugated beta actin Search Results


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GenScript corporation hrp-conjugated mouse β-actin antibody
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FUJIFILM horseradish peroxidase (hrp)-conjugated β-actin mab
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MBL Life science hrp-conjugated rabbit polyclonal ab specific β-actin
αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and <t>β-actin</t> are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.
Hrp Conjugated Rabbit Polyclonal Ab Specific β Actin, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+beta+actin/hrp+conjugated+anti+%CE%B2+actin+antibody/pmc03871693-84-98-102
Average 90 stars, based on 1 article reviews
hrp-conjugated rabbit polyclonal ab specific β-actin - by Bioz Stars, 2026-11
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ICN Biomedicals hrp-conjugated anti- β -actin antibody
αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and <t>β-actin</t> are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.
Hrp Conjugated Anti β Actin Antibody, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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KangChen Inc horseradish peroxidase- (hrp-) conjugated β -actin
αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and <t>β-actin</t> are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.
Horseradish Peroxidase (Hrp ) Conjugated β Actin, supplied by KangChen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hrp+conjugated+beta+actin/horseradish+peroxidase+++hrp+++conjugated+%CE%B2++actin/pmc03263562-33-41-47
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N/A
Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility,structure and integrity. Beta actin is a major constituent of the contractile apparatus and
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αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and β-actin are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.

Journal: PLoS ONE

Article Title: The Integrin-Linked Kinase-PINCH-Parvin Complex Supports Integrin αIIbβ3 Activation

doi: 10.1371/journal.pone.0085498

Figure Lengend Snippet: αIIbα6Bβ3-active parental cells were transiently transfected with PINCH siRNAs (p157 and p755) (A), α-parvin siRNAs (pa503 and pa761) (C), β-parvin siRNAs (pb900 and pb1011) (C), kindlin-2 siRNAs (k770 and k1733) (E), negative control siRNAs, and scrambled siRNAs. Cell lysates were electrophoresed on SDS-PAGE gels, and the separated proteins were immunoblotted with the indicated Abs. GAPDH and β-actin are shown as internal loading controls. The activation indexes of transfected cells (B, D, F) were calculated using the formula shown in Materials and Methods. A value of 100% implies the maximum PAC-1 binding to the cells treated with dithiothreitol (DTT). Data represent means ± standard deviation (SD) of three (B, F) or four (D) independent experiments. ** indicates P < 0.01.

Article Snippet: The membranes were incubated with either one of the following primary Abs: 0.125 μg/ml mouse mAb specific for ILK, 3/ILK (BD Biosciences), 0.25 μg/ml mouse mAb specific for PINCH, 49/PINCH (BD Biosciences), rabbit polyclonal Ab specific for α-parvin (IgG fraction; 1:3,000) (Sigma-Aldrich), 1 μg/ml mouse mAb specific for β-parvin, 11A5 (Millipore, Temecula, CA), mouse mAb specific for talin, 8D4 (ascites fluid; 1:2,000) (Sigma-Aldrich), rabbit polyclonal Ab specific for kindlin-2 (IgG fraction; 1:1,000) (ProteinTech Group, Chicago, IL), 0.5 μg/ml rabbit polyclonal Ab specific for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), FL-335 (Santa Cruz Biotechnology), horseradish peroxidase (HRP)-conjugated rabbit polyclonal Ab specific for β-actin (IgG fraction; 1:2000) (MBL, Woburn, MA), or 1 μg/ml mouse mAb specific for GFP, B-2 (Santa Cruz Biotechnology) for 90 minutes at room temperature.

Techniques: Transfection, Negative Control, SDS Page, Activation Assay, Binding Assay, Standard Deviation